I placed vinegar in the three substrates, pumice, gravel, and red volcanic rock and no bubbles on any of the 3. I dont understand why the red volcanic rock does not bring down the pH. My meter is calabrated and is 7.1 in 7.1 test solution. I have a medium fish load, I have 1 male Microgeophagus ramirezi boliviana and 5 females which have hatched 4 batches of fry. None of the fish die, most of which are from the amazon, 3 Britlenose ancetrus, 6 Otosinclus, 12 Corydoras, 5 Botia macracatha, 34 Cardinal tetra. I have several Echinodorus which are slowly dying. Its low light , natural light in the morning from greenhouse setting. My fishroom is a converted greenhouse.Thanks Jimmy