This site is supported by the advertisements on it, please disable your AdBlocker so we can continue to provide you with the quality content you expect.
  1. This site uses cookies. By continuing to use this site, you are agreeing to our use of cookies. Learn More.
  2. We are after as many aquarium plant images that we can get, doing so will assist us in completing the aquarium plant database.

    https://barrreport.com/threads/aquatic-plant-images-wanted.14374/
    Dismiss Notice
Cookies Aquarium Plant Images Wanted

N & P reference solutions

Discussion in 'CO2 Enrichment' started by PaulB, Apr 20, 2007.

  1. PaulB

    PaulB Subscriber

    Joined:
    Jan 24, 2005
    Messages:
    73
    Likes Received:
    0
    Local Time:
    4:43 AM
    i want to mnak up reference solutions to calibrate my NO3 & PO4 test kits. Does anyone know what quantity of KNO3 & KH2PO4 to use to make up base reference solutions with say 1lt of distilled water, which can then be diluted to make up 5, 10 & 20ppm NO3 solution and 1, 2 & 3ppm PO4 solution. :)
     
    #1 PaulB, Apr 20, 2007
  2. neil1973

    neil1973 Prolific Poster

    Joined:
    Dec 17, 2005
    Messages:
    58
    Likes Received:
    0
    Local Time:
    4:43 AM
    1.433g of KH2PO4 will give 1000ppm PO4 in 1 litre of water.

    1.631g of KNO3 will give 1000ppm of NO3 in 1 litre of water.

    Regards
    Neil
     
    #2 neil1973, Apr 20, 2007
  3. Tom Barr

    Tom Barr Founder
    Staff Member Administrator

    Joined:
    Jan 23, 2005
    Messages:
    18,662
    Likes Received:
    606
    Local Time:
    4:43 AM
    Make sure you store these solutions in very tight sealing bottles.
    Make what you need for a few times for the low concentrations.

    1000ppm can be diluted by using 1ml of the 1000ppm into 9 mls of DI water.
    Now you have 10mls of 100ppm NO3.

    Now take 5 mls of the 100ppm NO2 solution and add 5mls of DI water.
    Now you have:

    999mls of 1000ppm NO3 solution
    5 mls of 100ppm
    10 mls 50ppm.

    Take 1 ml of the 50ppm and add 9 mls of DI water, now you have 5ppm.

    You can make up whatever concentration you want by going from high concentration to low concentration and dilute with DI water.


    Regards,
    Tom Barr
     
    #3 Tom Barr, Apr 20, 2007
  4. Laith

    Laith Lifetime Charter Member
    Lifetime Member

    Joined:
    Jan 24, 2005
    Messages:
    182
    Likes Received:
    0
    Local Time:
    4:43 AM
    Here's what I use for NO3:

    - Mix 4.1g of KNO3 in 500ml of RO water.
    - 1ml of this solution in 1l of RO water should give you 5.03mg/l of NO3...

    For example, 4ml of the initial mix in 1l of RO water should give you 20.12mg/l of NO3.
     
    #4 Laith, Apr 20, 2007
(You must log in or sign up to post here.)
Loading...
Similar Threads - reference solutions
  1. Whitebeam

    Reference solutions for Ammonia and Nitrite?

    Whitebeam, Nov 20, 2010, in forum: Estimative Index
    Replies:
    5
    Views:
    81
    Biollante
    Nov 22, 2010
  2. Henry Hatch

    Reference solutions

    Henry Hatch, Jan 12, 2008, in forum: General Plant Topics
    Replies:
    1
    Views:
    36
    Tom Barr
    Jan 12, 2008
  3. Tom Barr

    How to make NO3 and PO4 reference solutions(repost from Left C)

    Tom Barr, Jul 18, 2007, in forum: Estimative Index
    Replies:
    35
    Views:
    1,920
    Briceboke
    Jul 2, 2018
  4. Richard McConchie

    Does bromothymol blue affect the reference solution's dKH?

    Richard McConchie, Jun 6, 2016, in forum: CO2 Enrichment
    Replies:
    3
    Views:
    155
    Justlikeapill
    Jul 5, 2016
  5. Tom Barr

    Correlating pH meter readings to Reference cell KH/pH measurements vs known reference

    Tom Barr, Aug 28, 2012, in forum: CO2 Enrichment
    Replies:
    8
    Views:
    121
    Forumsnow
    Sep 3, 2012

Share This Page