This site is supported by the advertisements on it, please disable your AdBlocker so we can continue to provide you with the quality content you expect.
  1. This site uses cookies. By continuing to use this site, you are agreeing to our use of cookies. Learn More.
  2. We are after as many aquarium plant images that we can get, doing so will assist us in completing the aquarium plant database.

    https://barrreport.com/threads/aquatic-plant-images-wanted.14374/
    Dismiss Notice

Lab Journal... Tissue Culture Startup

Discussion in 'Journals' started by adkaqua, May 25, 2018.

  1. adkaqua

    adkaqua Member

    Joined:
    May 7, 2018
    Messages:
    52
    Likes Received:
    33
    Local Time:
    10:13 AM
    I never read it. Didn't have the attention span. So my JP is movie JP.

    Same with MCU. Never read comics. So what the movies show is my MCU.

    Sent from my SM-N950U using Tapatalk
     
  2. adkaqua

    adkaqua Member

    Joined:
    May 7, 2018
    Messages:
    52
    Likes Received:
    33
    Local Time:
    10:13 AM
    No idea yet. Flourescent plants is far down the line. Cultivation is first and foremost. If genetic work never happens but I can make the business thrive growing and selling, I'll be happy. I've got a lot of ideas drafted up and backed with journal, reviewed work with the genes of interest, but that's a long ways out if it happens.

    Sent from my SM-N950U using Tapatalk
     
  3. Wobblebonk

    Wobblebonk Member

    Joined:
    Jul 3, 2018
    Messages:
    31
    Likes Received:
    21
    Local Time:
    11:13 AM
    Hah well if you want to get technical I believe MCU stands for Marvel cinematic universe so it doesn't really matter if you've read the comics!

    Regardless, I hope you are successful at this, ordering tissue cultures online has certainly been a crapshoot for me. Praying my nana snow white tissue culture arrives in good condition...
     
    adkaqua likes this.
  4. adkaqua

    adkaqua Member

    Joined:
    May 7, 2018
    Messages:
    52
    Likes Received:
    33
    Local Time:
    10:13 AM
    I haven't even seen it available in TC yet. I bought mine bare root. It's destined for TC in future though. Wish I'd gotten it in TC to start with, would be that much father ahead

    Sent from my SM-N950U using Tapatalk
     
  5. X3NiTH

    X3NiTH New Member

    Joined:
    Jul 3, 2018
    Messages:
    13
    Likes Received:
    10
    Local Time:
    4:13 PM
    I really hate to be cynical but If I were running a tissue culture business I'd be spiking the pots with a beneficial bacteria culture small enough that it doesn't cause problems for the plants in the pot (short term shelf life) but it does cause problems for replication attempts without expert knowledge and tools, which is the whole point of why you're going to so much trouble with the sterilisation process and finding it frustrating that the tissue culture pots you are getting in are not sterile (handling issues between supplier and vendor can't be to blame for all of the sterility issues your having).

    If it were easy to do TC then everyone would be doing it, I'd love to dabble in it for Buce by the bucket load.
     
  6. adkaqua

    adkaqua Member

    Joined:
    May 7, 2018
    Messages:
    52
    Likes Received:
    33
    Local Time:
    10:13 AM
    Ithink most of the contamination I'm seeing is from shipping from supplier to distrubutor or vendor from overseas. Then shipping to consumer. Issue with spiking your own containers is it will go crazy. Crazy. There is no beneficial to organisms when being grown in an ideal, steralized environment. You'll kill your plants as they're overgrown with what is usually beneficial. Businesses doing TC in competition with each other do just that, buy each other's stuff. Unless you're growing a trademarked cultivar, someone is gonna buy your sterile plants and do it themselves. Luckily I have enough experience to be able to handle some of it. Overall I lost less than I thought. Delicate plants that I aggressively steralized. Mosses, mayaca, etc. But I successfully established about 3/4 of what I started with initially. I'm actually moving on to plants from my grow out aquarium and steralizing them. It'll be a learning curve, trial and error, but that's what the big grow out tank is for. Then I can make sure I have backups when I inevitably kill some.

    Sent from my SM-N950U using Tapatalk
     
  7. adkaqua

    adkaqua Member

    Joined:
    May 7, 2018
    Messages:
    52
    Likes Received:
    33
    Local Time:
    10:13 AM
    Between established (recovering and in full multiplication mode), and current round of new species.. I've got 34 species in growth media

    Sent from my SM-N950U using Tapatalk
     
  8. adkaqua

    adkaqua Member

    Joined:
    May 7, 2018
    Messages:
    52
    Likes Received:
    33
    Local Time:
    10:13 AM
    I do think handling can be to blame for most of my issues rn, since I only purchased single cups of most of them. The ones I got multiples of, I managed to get going no prob. I like DHG, and my crypto pink panther. Had dupes so when one cup arrived open or leaking... I could use another. Had I afforded more of each to start I'd have had better luck. However, I'd already dropped 1800 on plants. I wasn't getting dupes haha

    Sent from my SM-N950U using Tapatalk
     
  9. adkaqua

    adkaqua Member

    Joined:
    May 7, 2018
    Messages:
    52
    Likes Received:
    33
    Local Time:
    10:13 AM
    One last injection of cash coming in. Took a little more than another grand out of my 401k. Buying 2 more growing racks, the necessary components to upgrade the t8 fixtures I bought to t5ho, mailing labels and printer, and the containers I'll be using to sell them in once I get the check. This is it. No more funds in reserve. This last bit is all the funds I'll have to work with and it's getting mostly consumed buying shelving and shipping supplies.



    I'm on track to have over 10k plantlets by August (looking like over 4k AR mini alone). And assuming contamination stays as low as I'm maintaining in my established cultures.... By the end of the year I could be at over 50k plantlets in culture. It's mind boggling. I don't know that I actually really thought I'd be able to do this. But I kept telling myself I could. I would. Been talking about having my own lab since 2009 when I decided to go back to college. And not only do I have one, but I've successfully gotten it on track and running well.




    "We're gonna need a bigger boat"

    Sent from my SM-N950U using Tapatalk
     
    jfhrtn and a1matt like this.
  10. adkaqua

    adkaqua Member

    Joined:
    May 7, 2018
    Messages:
    52
    Likes Received:
    33
    Local Time:
    10:13 AM
    Fast update. New pair of 55s. Exciting.

    Also, new media recipe is much better. Both containers planted same time. And same size. A month ago. Coming along. [​IMG][​IMG]

    Sent from my SM-N950U using Tapatalk
     
    a1matt and Phishless like this.
  11. jbs47

    jbs47 Lifetime Members
    Lifetime Member

    Joined:
    Mar 12, 2015
    Messages:
    80
    Likes Received:
    21
    Local Time:
    4:13 PM
    Those quantities are amazing...Eagerly following your quest ...may not comment but I am following. Best of luck, although your skill will make your own luck, i"m sure.
     
    adkaqua likes this.
  12. jokochimpa

    jokochimpa New Member

    Joined:
    Apr 24, 2018
    Messages:
    2
    Likes Received:
    1
    Local Time:
    8:13 AM
    Definitely have been following this thread. I find this absolutely fascinating. Watching your journey through this is a great read. I really hope this all works out as well as you envision this!
     
    adkaqua likes this.
  13. Tributary

    Tributary Junior Poster

    Joined:
    Aug 13, 2014
    Messages:
    5
    Likes Received:
    1
    Local Time:
    4:13 PM
    As a fellow Upstater (Finger Lakes!), I can't wait for you to open for business. Good luck!
     
    adkaqua likes this.
Loading...

Share This Page