Lab Journal... Tissue Culture Startup

Congratulations, you sure have your hands full now. Good luck

So cool what you have done...Autoclave should make things a little easier for you.

Long time lurker here. Super glad you were able to bounce back! Looking forward to more updates!


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Two days into my liquid media test, things are looking promising. Unfortunately, some plants I received were already contaminated in their tissue culture packaging, leading me to employ a rescue mission. One was lost in the process, but the rest underwent an isopropyl wash, bleach wash, rinsing, and were placed in my experimental media before their cubes succumbed to bacteria and fungus. The four I was concerned about turned out to be in bad shape, losing one from that batch. Additionally, I decided to salvage two cups of Glossostigma slated for disposal, which were heavily infected with bacteria. Despite a more aggressive sterilization protocol, I am cautiously optimistic after placing them on the orbital shaker in my low agar liquid media.

This experiment serves a dual purpose: testing the effectiveness of the media and protocol, and potentially saving the Glossostigma. Since Glosso can be affordable, the success of this method might extend to plants in my aquarium. Sterilizing submerged and terrestrial plant explants without harming them can be challenging. Thus, this test will not only determine the fate of two cups of Glosso but also refine the protocol for future use. In a week, I plan to transfer them back to a more solid media on the grow racks with the rest of the plants. With all my tools and glassware cleaned, I'm eager to resume multiplying after work tonight, aiming for another dozen or so.