I have some of this after redoing the garage tanks. I also have ample amounts of Rotala mexicana red and araguaia, A senegalensis, A pedicillata. the Erios and BV's do not count, they do not care.
I have 2-3 tanks available to torture said plants.
These serve as good control tanks for a good reason, they all grown these plants with little effort.
More replicates.
I can torture a few different ways.
tank 1: add more Fe, say 0.05, 0.2, 1.0 ppm as DTPA, or as CMS+B. I'll likely go with the CMS+B first.
tank 2: none
tank three, a different treatment level or a replicate. Tank 3 will have less light though.
I can alternate and repeat the test after 2-4 weeks to the other tanks, one set as a no dose control: now gets dose and the prior treated tank, no dosing.
So while I lack the replicates being tested at the exact same time, I can do something called repeated measures. Which act like more replicates than say the two tanks. So if you do this 3 x, you now have say 6 replicates, enough to do statistical probability.
The key here is to be able to grow the plants nice and robustly to begin with.
Without that, you cannot be certain there are no dependent factors. Even here.........but..........the likelihood is extremely high there are not any.
the hypothesis is negative plant will occur if I add 0.2ppm or higher Fe dosing and positive if I dose 0.05 and no change or negative growth without dosing any.
The test after this includes the macro dosing along with traces.
This is a lot of work, if..........if....I was not sure I could not recover these plants easily and grow them well already. So it's not entirely out of my way from what I am doing already. Dosing and noting the effects is rather easy.
I have 2-3 tanks available to torture said plants.
These serve as good control tanks for a good reason, they all grown these plants with little effort.
More replicates.
I can torture a few different ways.
tank 1: add more Fe, say 0.05, 0.2, 1.0 ppm as DTPA, or as CMS+B. I'll likely go with the CMS+B first.
tank 2: none
tank three, a different treatment level or a replicate. Tank 3 will have less light though.
I can alternate and repeat the test after 2-4 weeks to the other tanks, one set as a no dose control: now gets dose and the prior treated tank, no dosing.
So while I lack the replicates being tested at the exact same time, I can do something called repeated measures. Which act like more replicates than say the two tanks. So if you do this 3 x, you now have say 6 replicates, enough to do statistical probability.
The key here is to be able to grow the plants nice and robustly to begin with.
Without that, you cannot be certain there are no dependent factors. Even here.........but..........the likelihood is extremely high there are not any.
the hypothesis is negative plant will occur if I add 0.2ppm or higher Fe dosing and positive if I dose 0.05 and no change or negative growth without dosing any.
The test after this includes the macro dosing along with traces.
This is a lot of work, if..........if....I was not sure I could not recover these plants easily and grow them well already. So it's not entirely out of my way from what I am doing already. Dosing and noting the effects is rather easy.